Gamora: I'm a warrior, an assassin. I don't dance.
Peter Quill: Really? Well, on my planet, we have a legend about people like you. It's called Footloose. And in it, a great hero, named Kevin Bacon, teaches an entire city full of people with sticks up their butts that, dancing, well, is the greatest thing there is.
Gamora: ...Who put the sticks up their butts?

Tony Stark: [in the Hulkbuster] Listen to me, that little witch is messing with your mind. You're stronger than her, you're smarter than her. You're Bruce Banner!
Tony Stark: Right, don't mention puny Banner...

Vicki Vale: [distracting Joker] Mr. Joker, you make such beautiful things... oh, you're so powerful, and... PURPLE! Oh, I love purple.
Batman: Excuse me.
[Joker looks]
Batman: Have you ever danced with the devil by the pale moon light?
[punches him]

Like the jacket? It keeps me safe when I'm... jogging at night!

The Riddler

I'm going to tear you apart... from the inside!

Ultron

[finds out Peter is Spider-Man] Oh, I'm in trouble.

Gwen Stacy

Secrets have a cost. They're not free. Not now, not ever.

May Parker

Peter? I know things have been difficult lately and I'm sorry about that. I think I know what you're feeling. Ever since you were a little boy, you've been living with so many unresolved things. Well, take it from an old man. Those things send us down a road... they make us who we are. And if anyone's destined for greatness, it's you, son. You owe the world your gifts. You just have to figure out how to use them and know that wherever they take you, we'll always be here. So, come on home, Peter. You're my hero... and I love you!

Ben Parker

[on the Winter Soldier] He's fast... strong... and has a metal arm.

Steve Rogers

Tony Stark: [Tony has just been told by a US Marshal that tomorrow he has to attend court in front of the US Armed forces committee] Show me the badge.
Happy Hogan: [to the US Marshall] He likes the badge.
U.S. Marshal: [She shows her badge] Still like it?
Tony Stark: Yeah.

You have until tomorrow to assemble my missile.

Raza

Yinsen: [gazing at a helmet] That doesn't look like a missile... What are you building, Stark?
Tony Stark: I'm working on something big.

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